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Monkeypox Virus MPV Nucleic Acid Detection Kit Real Time PCR Method

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Monkeypox Virus MPV Nucleic Acid Detection Kit Real Time PCR Method

Monkeypox Virus MPV Nucleic Acid Detection Kit Real Time PCR Method
Monkeypox Virus MPV Nucleic Acid Detection Kit Real Time PCR Method
Monkeypox Virus MPV Nucleic Acid Detection Kit Real Time PCR Method Monkeypox Virus MPV Nucleic Acid Detection Kit Real Time PCR Method Monkeypox Virus MPV Nucleic Acid Detection Kit Real Time PCR Method Monkeypox Virus MPV Nucleic Acid Detection Kit Real Time PCR Method Monkeypox Virus MPV Nucleic Acid Detection Kit Real Time PCR Method

Large Image :  Monkeypox Virus MPV Nucleic Acid Detection Kit Real Time PCR Method

Product Details:
Place of Origin: China
Brand Name: Renji Medical
Certification: CE, SGS, SDS, MA, YBTC
Model Number: RJ-M-1001
Payment & Shipping Terms:
Minimum Order Quantity: 5000 Pieces
Price: Negotiable
Packaging Details: 10 sets per box, 50 boxes in a carton
Payment Terms: T/T
Supply Ability: 200K pieces per day

Monkeypox Virus MPV Nucleic Acid Detection Kit Real Time PCR Method

Description
Standard: Reliable Test Kit Certificated By CE Mode: Self-test In Home
Packing: 50 Tests/kit Test Purpose: Used To Detect An Active Infection
Certification: CE, SGS, ISO13485 Feature: High Sensitivity
Save: Keep Dry Precautions: Biological Risks
High Light:

MPV Nucleic Acid Detection Kit

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Monkeypox Virus MPV Detection Kit

Monkeypox Virus(MPV) Nucleic Acid Detection Kit (Real-Time PCR Method)

››› World’s Fastest Covid Testing Kit!

››› Easy operation, Safe and accurate, Money-saving!!

 

【PRODUCT NAME】

Monkeypox Virus (MPV) Nucleic Acid Detection Kit (Real-time PCR Method)

 


【Package Specification

50 tests/kit & 200 tests/kit

 

Intended Use

This kit is used forthe detection of Monkeypox Virus(MPV) in serum or lesion exudate samples by using real
time PCR systems.

 

【Test Principle】
The principle of the real-time detection is based on the fluorogenic 5’nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an  increase in the fluorescence signal is detected initially is proportional to the amount of the specific PCR product. Monitoring the fluorescence intensities in real-time allows the detection of the accumulating product without having to re-open the reaction tube after the amplification. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virus through polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems´ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel FAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the Cy5 fluorescence of the internal control (IC). An external positive control defined as 1×10 7copies/ml is supplied which allow the determination of the gene load.

 

【Components of the Diagnostic Kit】

Monkeypox Virus MPV Nucleic Acid Detection Kit Real Time PCR Method 0

 

Monkeypox Virus MPV Nucleic Acid Detection Kit Real Time PCR Method 1

 
Note: 1. Do not mix or exchange components from different kit lots.
2.The MPV Negative Control is sterile nuclease-free water and Internal Control(IC) gene, and the MPV Positive Control is DNA in vitro which contains target genes and Internal Control(IC) gene.
 
【Storage and Stability】
1. The diagnostic kit should be stored in a sealed pouch at -20 ± 5℃. The expiry date is 12 months.
2. Please refer to the date of manufacture and expiry date on the outer package.
3. The reagent keep valid and stable within the expiry date if not used. The kit should not be frozen and thawed more than 5 times.

 

【Applicable Instrument】
The diagnostic kit is applicable to MA-6000, ABI series, Bio-Rad series, Roche LightCycler R480, Cepheid
SmartCycler, Rotor-Gene series and other multi-channel real-time quantitative PCR instruments.

 

【Specimen Requirements】
1.Sample types: serum, or lesion exudates samples

 

2.Specimens can be extracted immediately or frozen at -20°C to -80°C.

 

3.Transportation of clinical specimens must comply with local regulations for the transport of etiologic agents.

 

 

【The Sample Result Judgment 】
1.Ifthe test sample detects a typical S-type amplification curve in the FAM≤38, and Cy5 has a typical amplification curve, theCtis≤38.the samplecanbe judgedtobe MPVpositive.

2.If the test sample has no Ct or Ct > 38 in the FAM channels, and there is a typical S-type amplification curve in the Internal Control channel(Cy5), the Ctis ≤38.the sample canbe judgedtobeMPVnegative.

3.If the test sample have no typical S-type amplification curve (No Ct value) or Ct value> 38 is detected in the FAM, andCy5 channel does not have a typical amplification curve (No Ct value) or Ct value> 38, it means that there is a problem with the quality of the sample or a problem with the operation. If the result is invalid, you should find and eliminate the cause,collectthe sampleagain, andrepeatthe test(ifthe test resultis   stillinvalid,please contact the company).

 

【Limitations of Detection Method】
1.Test results of the diagnos tickit can be used only for clinic reference. The clinical diagnosis and treat ment of patients should be considered in conjunction with their symptoms, signs,medicalhistory and other related conditions.

2.False negative results may occur when the concentration of the detecte dnucleicacid in the test sampleis below the minimumde tection limit of this kit.

3.Improperhandlingofthetestedsampleduringcollection,transportation, storage, andprocessingcaneasily resultin DNA degradation and falsenegative results.

4.When samples are cross-contaminated during collection,transportation, storage, and rocessing,it is easy togetfalse positive results.

 

 

 

Monkeypox Virus MPV Nucleic Acid Detection Kit Real Time PCR Method 2

 

【Product Performance Index】
1.LOD:The limitofdetectionis200copies/ml. 2. Precision:Coefficientof variation(CV%)ofprecisionCtvaluewithinbatch≤3%. 3.Specificity:Thereisnocross reactionbetweenthekit andpositive samples, suchasSmallpoxVirus,CowpoxVirus, VacciniaVirus etc.

 

【Precations】
1.The entire detection process should be performed strictly in accordance with there quirements of this manual in the reagent preparation area, sample processing area, and PCR amplification area, and the experimental clothes,instruments, and consumables ineacharea should be used in dependently and cannotbe mixed.

2.Negative andpositive controls shouldbe setfor each experiment.

3.All reagents in the kit should be fully thawed and mixed at room temperature and centrifuged immediately before use.

4.All negative and positive controls in the kit should be transferred to the sample preparation area and stored separately before the firstuse.

5. To prevent fluorescence interference, avoid touching the PCR reaction tube directly with bare hands, and avoid any marking on the PCR reaction tube.

6. The instrument amplification related parameters should be set in accordance with the relevant requirements of this manual, and differentbatchesof reagents cannotbemixed.

7.The product waste during the experiment should be detoxified before being discarded.

 

【Index of Symbol】

 

Monkeypox Virus MPV Nucleic Acid Detection Kit Real Time PCR Method 3
 
FACTORY VIEW

Sterile Pipette Tips, Filtered, DNAse and RNAse Free, Autoclavable - 200 µl 4Sterile Pipette Tips, Filtered, DNAse and RNAse Free, Autoclavable - 200 µl 3

 

FACTORY WORKSHOP

Sterile Pipette Tips, Filtered, DNAse and RNAse Free, Autoclavable - 200 µl 5

EXPORTER

 

Magnus Internationa Limited

 

F12, New City Internationa Mansion A, 234 Huapao Ave.

Liuyang, Hunan Province 410300 China

 

Contact: Goodwellmedical@gmail.com

 

AUTHORIZED REPRESENTATIVE

 

Lotus NL B.V.

Koningin Julianaplein 10, 1e Verd, 2595AA, The Hague, Netherlands.

 

PRODUCT AND STANDARDS

Sterile Pipette Tips, Filtered, DNAse and RNAse Free, Autoclavable - 200 µl 8

Sterile Pipette Tips, Filtered, DNAse and RNAse Free, Autoclavable - 200 µl 7

 

 
 
 
 
Sterile Pipette Tips, Filtered, DNAse and RNAse Free, Autoclavable - 200 µl 10
Sterile Pipette Tips, Filtered, DNAse and RNAse Free, Autoclavable - 200 µl 9
SHIPPING
Sterile Pipette Tips, Filtered, DNAse and RNAse Free, Autoclavable - 200 µl 11
 
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